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    2. 天然羥基磷灰石圓盤狀試樣的制備及細胞在其表面的培養
      來源: | 作者: | 發布時間:2014-4-15 15:59:05

      天然羥基磷灰石圓盤狀試樣的制備及細胞在其表面的培養
      王健丹,張志偉,呂曉迎
      (東南大學生物電子學國家重點實驗室,南京,210096)
      摘要:本研究的目的是研究天然羥基磷灰石的生物相容性。首先采用高溫煅燒法從豬骨中制備天然羥基磷灰石,然后壓制成圓盤狀試樣。接著,在體外培養小鼠骨髓間充質干細胞,并檢測其細胞活力代謝標準曲線,最后對小鼠間充質干細胞在NHA圓盤狀試樣表面的生長狀況進行研究。結果顯示650°C煅燒能夠從豬骨中獲得NHA,骨髓間充質干細胞的活力代謝標準曲線的研究結果表明適宜的細胞種板濃度為6000細胞/孔;細胞在NHA圓盤狀試樣表面生長狀態良好,這表面NHA具有良好的生物相容性。
      關鍵詞:生物材料;天然羥基磷灰石;間充質干細胞;細胞活力代謝標準曲線;生物相容性
      中圖分類號:R318.08

       Preparation of Disk-like Natural Hydroxyapatite Samples and Cell Culture on Their Surfaces
      WANG Jiandan, ZHANG Zhiwei, Lü Xiaoying
      (State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096)
      Foundations: Doctoral Program of Higher Education of China (No.20100092110027); 973 Project (No. 2009CB930000); the National Natural Science Foundation of China (No. 31170910, 31271012, 30770583); the Graduate Research and Innovation Program of Jiangsu Province (No. CX10B-082Z) Brief author introduction:王健丹(1987-),女,碩士,主要研究方向:生物材料,生物相容性 Correspondance author: 呂曉迎(1956-),女,教授,主要研究方向:生物材料,生物相容性,生物電子學.

      Abstract: The purpose of this study is to prepare natural hydroxyapatite (NHA) for biocompatibility study. The NHA was prepared from pig bones by means of a calcining procedure, and then were made into disk-like samples. Subsequently mouse mesenchymal stem cells (MSCs) were cultured in vitro and the cell viability metabolism standard curve was obtained. Finally the mouse MSCs were also tried to seed on the surface of disk-like NHA samples. The result showed NHA could be derived from pig bones by a 650°C calcining procedure. MSCs viability metabolism standard curve was obtained and proper cell density of 6000 cells per wells was selected. The cells grew well on the surface of disk-like NHA samples. These results demonstrated that NHA have good biocompatibility.
      Key words: Biomaterial; Natural hydroxyapatite (NHA); Mesenchymal stem cells (MSCs); Cell  viability metabolism standard curve; Biocompatibility

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