摘要：遠端小管和入球小動脈之間的直接接觸已經在大鼠和家兔報道過。除了致密斑外，該反饋機制的存在對全身酸堿和電解質狀態的控制也有顯著影響。在本研究中，我們應用計算機輔助三維腎追蹤結合免疫組化方法研究在小鼠腎臟中是否存在類似的接觸現象。在共168個追蹤的腎單位中，大多數腎單位（短髓袢的約90％、長髓袢腎單位的有75 ％）有這種入球小動脈和遠端小管之間的直接接觸。這些接觸部位總是發生在遠曲小管的最后10 ％節段。值得注意的是，在接觸部位的腎小管管壁上總能找到閏細胞，而且是nonA-nonB 類型的。閏細胞在遠曲小管的這種密切聯系表明，這些接觸部位可能存在和致密斑處類似的調控機制，可能參與在后致密斑腎小管節段上的pH 值和電解液的濃度調控。
關鍵詞：關鍵詞1 組織胚胎學；關鍵詞2 數字化三維重建；關鍵詞3 小鼠腎臟
Direct Physical Contact between Intercalated Cells in Distal Convoluted Tubule and the Afferent Arteriole in Mice#
REN Hao, Xiaoyue Zhai
(china medical university, histology 5 and embryology）
Foundations:Specialized Research Fund for the Doctoral Program of Higher Education (No. 20102104120020)
Brief author introduction:associate prof.
Abstract: IDirect physical contact between the distal tubule and the afferent arteriole has been reported in rats and rabbits. The existence of a feedback mechanism termed cross-talk similar to that at macular densa has been proposed, which has significant impact on the control of systemic acid-base and electrolytes status. In the present study, we applied computer assisted 3D nephron tracing combined with immunohistochemistry to investigate such contacts in mice. With tracing, it became possible to determine whether the tubule and the contacting arteriole were associated to the same parent nephron
or not. We investigated a total of 168 nephrons from 3 mouse kidneys. The majority of the nephrons (approximately 90% of the short-loop and 75% of the long-loop nephrons) had direct physical contact between their own afferent arteriole and distal tubule. These contacts always occurred at the last 10% of the distal convoluted tubule. Type nonA-nonB intercalated cells were always found in the tubular wall at the contact site. In conclusion, the present work demonstrated the existence of direct
tubule-arteriole contact in mice. The close association of intercalated cells in the distal convoluted tubule suggests that these contacts may be the morphological basis for a cross-talk mechanism, which is potentially involved in the post-macula densa humeral pH and electrolyte regulation in the kidney.
Key words: histology and embryology; 3D digital reconstruction; mouse kidney
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